In 1630s Moldavia, Asa Vajda, a vampiric witch, and her paramour, Javutich,[a] are sentenced to death for sorcery by Asa's brother Griabi. Asa vows revenge, and puts a curse on Griabi's descendants. Bronze masks with sharp spikes on the inside are placed over Asa and Javutich's faces and hammered into their flesh, but a sudden storm prevents the villagers from burning them at the stake.

Two centuries later, Dr. Choma Kruvajan and his assistant, Dr. Andrej Gorobec, are traveling through Moldavia en route to a medical conference when one wheel on their carriage breaks. While waiting for their coachman to fix it, the two wander into a nearby ancient crypt and discover Asa's tomb. Observing her death mask through a glass panel, Kruvajan breaks the panel (and the cross above it) by accident while striking a bat. He removes Asa's death mask, revealing a partially preserved corpse. He cuts his hand on the broken glass and some of his blood drips onto Asa.

Returning outside, Kruvajan and Gorobec meet Katia Vajda. She tells them she lives with her father and brother Constantine in a nearby castle that the villagers believe is haunted. Struck by her haunting beauty and sadness, Gorobec becomes smitten with Katia. The two men leave her and drive to an inn. Meanwhile, Kruvajan's blood brings Asa back to life. She contacts Javutich telepathically. He rises from his grave and goes to Prince Vajda's castle, where Vajda holds up a crucifix to ward off the reanimated corpse. However, Vajda is so terrified by the visit he becomes paralyzed with fear. Constantine sends a servant to fetch Dr. Kruvajan, but the servant is killed before he can reach the inn. Javutich brings Kruvajan to the castle under the pretext that his services are needed. Javutich leads Kruvajan to Asa's crypt. The witch hypnotizes Kruvajan and says she needs the rest of his blood. Asa then kisses him, turning him into her servant. By Asa's command, Kruvajan follows up on the request to tend to Vajda. He orders the crucifix removed from the room, ostensibly so it will not upset Vajda; this allows Javutich to return later and murder him.

Asa's plan is to revive herself by draining Katia of her life, since Katia is physically Asa reincarnated. Puzzled to hear that Kruvajan abandoned his patient shortly before he died, Gorobec questions Sonya, a little girl who saw Javutich take Kruvajan to the castle. She identifies Kruvajan's escort with a painting of Javutich. A priest and Gorobec go to Javutich's grave and find Kruvajan's body inside the coffin. Realizing he is now one of the undead, they kill him by driving a nail through his eye.

Javutich throws Constantine into a death pit and takes Katia to Asa. Asa drains Katia of her youth. When the witch goes to take her blood, the cricifix around Katia's neck thwarts her. Gorobec enters the crypt to save Katia, but Javutich attacks him and pushes him to the edge of the death pit. Constantine uses the last of his strength to pull Javutich into the pit and push Gorobec to safety. Gorobec finds Asa and Katia. Asa pretends to be Katia and tells Gorobec that Katia is the witch. Accordingly, he goes to kill Katia but notices the crucifix she is wearing has no effect on her. He turns to Asa and opens her robe, revealing a fleshless skeletal frame. The priest then arrives with many torch-carrying villagers, and they burn Asa to death. Katia awakens from her stupor, her life and beauty restored and is reunited with Gorobec.

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